Protein Purification Reagents and Kits
Biotium CD45RA (Leukocyte Marker)(K4B5), CF594 conjugate, 0.1mg/mL
Recognizes a protein of 205 kDa-220 kDa, identified as CD45RA. CD45RA is isoforms of the human leukocyte common antigen (CD45). Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. This antibody reacts with ABC and BC isoforms. CD45RA is expressed on 40-50% of peripheral CD4 T-cells, 50% of peripheral CD8 T-cells, B-cells, and leukemic B-cell lines. T-cells expressing CD45RA are naive or virgin T-cells. T-cells expressing CD45RO are memory T-cells. CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral T-cells. This MAb is useful in study on the subpopulation of CD4 or CD8 T-cells. It can especially be used to differentiate T-cell lymphomas (CD45RO ve) from B cell lymphomas (CD45RA ve). Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other l
Biotium CD62L (L-Selectin)(LAM1-116), CF740 conjugate, 0.1mg/mL
Selectins, also designated CD62 antigens, comprise a family of carbohydrate-binding proteins involved in mediating cellular interactions with leukocytes. L-Selectin (also designated LECAM-1 or CD62L) is expressed on the majority of B and naive T cells and on most monocytes, neutrophils and eosinophils. L-Selectin interacts with specific carbohydrates expressed by activated endothelial cells. P-Selectin (also designated GMP-140 or CD62P), expressed on activated platelets and endothelial cells, and E-Selectin (also designated ELMA-1 or CD62E), expressed on endothelial cells, exhibit overlapping ligand specificities. Both recognize sialyl-Le (x) as a ligand and bind to specific carbohydrates on neutrophils and monocytes. Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecti
Sino Biological Recombinant Human CD8 alpha Protein (APC site-specific conjugation, His Tag) 25
A DNA sequence encoding the Human CD8A (NP_001759.3) (Met 1-Asp 182) was expressed with a C-terminal polyhistidine tag. The protein is site-specifically conjugated with APC (Excitation Max.= 651 nm, Emission Max.= 662 nm).
STEMCELL Technologies RoboSep™ APC Positive Selection Kit II, For processing: 1 x 10^9 cells
The EasySep™ APC Positive Selection Kit II is designed to isolate cells that are labeled with APC (allophycocyanin)-conjugated antibodies by positive selection. Desired cells are targeted with antibody complexes recognizing APC and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.This product replaces the EasySep™ APC Positive Selection Kit (Catalog #18453) for even faster cell isolations.
STEMCELL Technologies RoboSep™ Human Naïve Pan T Cell Isolation Kit, For processing: 1 x 10^9 cells
The EasySep™ Human Naïve Pan T Cell Isolation Kit is designed to isolate naïve pan T cells from fresh or previously frozen human peripheral blood mononuclear cells or washed leukapheresis samples by immunomagnetic negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.
STEMCELL Technologies RoboSep™ Human Pan-Granulocyte Isolation Kit, For processing: 1 x 10^9 cells
The EasySep™ Human Pan-Granulocyte Enrichment Kit is designed to isolate granulocytes (eosinophils, basophils and neutrophils) from normal peripheral whole blood by negative selection. Undesired cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-granulocytes and magnetic particles. The labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.
STEMCELL Technologies RoboSep™ Human Gamma/Delta T Cell Isolation Kit, For processing: 1 x 10^9 cells
The EasySep™ Human Gamma/Delta T Cell Isolation Kit is designed to isolate gamma/delta T cells from fresh or previously frozen peripheral blood mononuclear cells by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-gamma/delta T cells and dextran-coated magnetic particles. The labeled cells are separated using the EasySep™ magnet without the use of columns. The desired cells are poured off into a new tube. To obtain a custom kit optimized for culture-expanded PBMCs, contact STEMCELL Technologies’ Technical Support at techsupport@stemcell.com.
STEMCELL Technologies RoboSep™ Rat T Cell Isolation Kit, For processing: 1 x 10^9 cells
The EasySep™ Rat T Cell Isolation Kit is designed to isolate T cells from single-cell suspensions of splenocytes or lymph nodes or from whole blood by negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.
STEMCELL Technologies Easy 250 EasySep™ Magnet, For isolating: 1.25 x 10^10 cells
Scale up your manual cell isolations from large-volume samples, such as leukopaks and whole blood. Rather than splitting your cell suspension and performing multiple rounds of isolations, with the Easy 250 EasySep™ magnet you can process up to 225 mL and 1.25 x 1010 cells in a single isolation.Use the Easy 250 EasySep™ magnet with a standard T-75 cm2 cell culture flask and EasySep™ reagents to separate cells from large-volume samples without the use of columns. With its strong, high-gradient internal magnetic field, the magnet isolates cells labeled with magnetic EasySep™ particles, allowing you to simply pipette out the unlabeled cell fraction and obtain cells that are immediately ready for downstream applications. Save time and obtain highly purified cells with EasySep™ column-free technology. Learn more about how EasySep™ technology works or get an overview of our other magnets on our EasySep™ Cell Separation Magnets page.
STEMCELL Technologies EasySepTM Human CD4CD127
Large-scale immunomagnetic cell isolation kit using particle release technology - 1 x 10.10 cells
IPHASE PHARMA SERVICES LLC Human Pan B Cells Negative Isolation Kit,10 test
This product adopts the immunomagnetic bead negative isolation method, using a biotinylated antibody mixture to bind to other cells in PBMC (human peripheral blood mononuclear cells) except the target cells, and then incubate with magnetic beads, and then under the action of an applied magnetic field, the other cells are retained in the magnetic field, and the target cells can be isolated. The isolated target cells do not combine with magnetic beads or antibodies and are suitable for subsequent experiments such as cell culture.
STEMCELL Technologies EasySep™ HLA Chimerism Whole Blood CD66b Positive Selection Kit, For processing: 60 mL whole blood processed
The EasySep™ HLA Chimerism Whole Blood CD66b Positive Selection Kit is designed to isolate highly purified CD66b+ cells from fresh whole blood by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD66b and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD66b antigen is expressed on mature granulocytes. Isolated cells are immediately available for downstream applications such as flow cytometry, culture, or DNA/RNA extraction for lineage-specific chimerism analysis.This product replaces the EasySep™ Human Whole Blood CD66b Positive Selection Kit (Catalog #18682) for even faster cell isolations.
Miltenyi Biotec, Inc. CD14 MicroBeads UltraPure, human
CD14 MicroBeads UltraPure can be used for the positive selection or depletion of human monocytes and macrophages from cord blood or PBMCs. They are particularly suited to work with low quality starting material, i. e., older buffy coats.
Cytek Biosciences Inc Hu CD4 T Cell Kit Amnis
Product Name: Muse Human CD4 T Cell Kit
Cytek Catalog Number: MIM100101
Product Class: Guava Muse Reagent
Type: Muse
Size: 100 Tests
STEMCELL Technologies Easy 50 EasySep™ Magnet, For isolating: 1 Unit
The Easy 50 EasySep™ Magnet is designed for cell separation procedures using selected EasySep™ reagents when processing a large number of cells (up to 4 x 10^9 cells). The Easy 50 EasySep™ Magnet generates a high-gradient magnetic field in the interior cavity that is strong enough to separate cells labeled with magnetic EasySep™ particles without the use of columns. The Easy 50 EasySep™ Magnet is designed to hold a standard 50 mL conical tube.